Interestingly, the AMPK1 T19A, and combined T19A and S40A (2A) mutations blocked all 32P incorporation at both T19 and S40, possibly indicating that lack of T19 phosphorylation precludes S40 phosphorylation, but not vice versa (Fig.?2F). in promoting proper chromosomal alignment, as loss of AMPK activity leads to misaligned chromosomes and … Continue reading Interestingly, the AMPK1 T19A, and combined T19A and S40A (2A) mutations blocked all 32P incorporation at both T19 and S40, possibly indicating that lack of T19 phosphorylation precludes S40 phosphorylation, but not vice versa (Fig